Peptide Dosage Calculator

BPC-157 typically comes in 5 mg lyophilized (freeze-dried) vials. To reconstitute, draw 2 mL of bacteriostatic water into an insulin syringe or mixing syringe. Slowly inject the BAC water down the inside wall of the vial — do not spray it directly onto the powder. Gently swirl (never shake) until the powder dissolves completely. This gives you a concentration of 2.5 mg/mL.

A common research dose of BPC-157 is 250–500 mcg per day, injected subcutaneously near the site of interest. At 2.5 mg/mL, a 250 mcg dose = 0.10 mL = 10 units on a U-100 syringe. Use the calculator above to determine your exact draw volume.

Store the reconstituted vial in the refrigerator at 2–8°C and use within 3–4 weeks. You can purchase high-quality BPC-157 from Fierce Longevity.

The amount of BAC water you add determines the concentration of your solution, which in turn determines how much liquid you draw per dose. There is no single "correct" amount — it depends on your vial size and target dose.

A good rule of thumb: choose an amount of BAC water that makes your per-dose draw volume fall between 5 and 50 units on your syringe. This range is easy to measure accurately. For example:

5 mg vial + 2 mL BAC water = 2.5 mg/mL concentration. A 250 mcg dose = 10 units.
10 mg vial + 2 mL BAC water = 5 mg/mL concentration. A 250 mcg dose = 5 units.
10 mg vial + 1 mL BAC water = 10 mg/mL concentration. A 500 mcg dose = 5 units.

If your calculated draw volume is less than 5 units, use less BAC water. If it exceeds your syringe capacity, use more BAC water.

Semaglutide for weight loss is typically administered as a once-weekly subcutaneous injection. The standard titration protocol starts low and increases gradually to minimize side effects:

Weeks 1–4: 0.25 mg/week
Weeks 5–8: 0.5 mg/week
Weeks 9–12: 1.0 mg/week
Weeks 13–16: 1.7 mg/week
Week 17+: 2.4 mg/week (maintenance dose)

Always follow your prescribing physician's guidance for your specific protocol. Compounded Semaglutide typically comes in 3 mg or 5 mg vials. Use the calculator above to determine your exact draw volume for each weekly dose.

You can get started with Semaglutide through Fierce Health's clinician-led program.

The CJC-1295 / Ipamorelin blend is one of the most popular growth hormone secretagogue combinations. It is typically dosed at 100–300 mcg of each peptide, 2–3 times per day, injected subcutaneously. Many protocols call for dosing before bed and/or first thing in the morning on an empty stomach.

Common vial sizes for the blend are 5 mg or 10 mg total. Reconstitute with 2 mL of BAC water for a convenient concentration. A typical dose of 300 mcg from a 5 mg vial reconstituted with 2 mL = 12 units on a U-100 syringe.

Purchase the CJC-1295 / Ipamorelin blend from Fierce Longevity.

A standard U-100 insulin syringe holds 1.0 mL total, divided into 100 equally spaced unit markings. Each unit equals exactly 0.01 mL. The markings are printed on the barrel of the syringe.

10 units = 0.10 mL
25 units = 0.25 mL
50 units = 0.50 mL
100 units = 1.00 mL

For very small doses (under 10 units on a U-100), consider using a U-30 syringe (0.3 mL, 30 units). The markings are more spread out, making it much easier to measure 2–5 unit doses accurately. A U-50 syringe (0.5 mL, 50 units) is a good middle ground.

Always read the syringe at eye level, with the needle pointing up, after tapping out any air bubbles.

Once reconstituted with bacteriostatic water, most peptides remain stable for 3–4 weeks when stored properly in the refrigerator at 2–8°C (36–46°F). Some peptides like BPC-157 and TB-500 are particularly stable and may last up to 4–6 weeks.

Key storage rules: Always keep the vial upright in the refrigerator. Never freeze a reconstituted vial. Avoid repeated temperature fluctuations. Use an alcohol swab to clean the rubber stopper before each draw to prevent contamination.

If the solution becomes cloudy, discolored, or develops particles, discard it immediately regardless of how long it has been stored.

TB-500 (Thymosin Beta-4) is commonly used for recovery and tissue repair. A typical protocol involves two phases:

Loading phase (weeks 1–4): 2–5 mg per week, split into 2–3 injections. For example, 2.5 mg twice per week.
Maintenance phase (weeks 5+): 2–5 mg every 2 weeks, or as needed.

TB-500 typically comes in 5 mg vials. Reconstitute with 2 mL of BAC water for a 2.5 mg/mL concentration. A 2.5 mg dose = 1.0 mL = 100 units (a full U-100 syringe). For smaller doses, use less BAC water or split across injections.

TB-500 can be injected subcutaneously anywhere — it is systemically active and does not need to be injected near the injury site. Purchase from Fierce Longevity.

The number refers to the total capacity of the syringe in units (and milliliters):

U-100 (1.0 mL): The most common insulin syringe. 100 unit markings across 1 mL. Best for doses between 10–100 units. Each small tick = 2 units on most brands.
U-50 (0.5 mL): 50 unit markings across 0.5 mL. Better precision for doses between 5–50 units. Each small tick = 1 unit.
U-30 (0.3 mL): 30 unit markings across 0.3 mL. Best precision for very small doses under 15 units. Each small tick = 0.5 units.

The key principle: use the smallest syringe that can hold your dose. Smaller syringes have more spread-out markings, making it easier to measure precisely. If your calculated dose is 5 units, a U-30 syringe will be far more accurate than a U-100.

The number of doses per vial depends on two things: the total peptide in the vial and your dose per injection. The formula is simple:

Doses per vial = Total peptide (mg) ÷ Dose per injection (mg)

For example, a 5 mg vial of BPC-157 at 250 mcg (0.25 mg) per dose = 20 doses. At one injection per day, that vial lasts 20 days. At 500 mcg per dose, it lasts 10 days.

The calculator above automatically computes this for you, along with how many weeks the vial will last at your chosen injection frequency. Use the Dose Schedule Planner in the results panel to compare different frequencies.

Quality matters enormously with peptides. Look for suppliers that provide third-party Certificates of Analysis (COAs), use USP-grade ingredients, and have transparent sourcing.

For GLP-1 weight loss peptides (Semaglutide, Tirzepatide), I recommend Fierce Health. They are a US-based, clinician-led telehealth company that provides medically supervised GLP-1 programs with real physician oversight. Their Semaglutide and Tirzepatide programs include the consultation, prescription, and medication shipped directly to your door.

For research peptides (BPC-157, TB-500, CJC-1295, Ipamorelin, Epithalon, and others), I recommend Fierce Longevity. All of their peptides are USP-regulated and verified by third-party testing with COAs available. Use code WARREN at checkout for a discount — or use my link which applies it automatically.